2ISJ
BluB bound to oxidized FMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2006-06-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9919 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.149, 172.724, 92.063 |
| Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.21 |
| Rwork | 0.207 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.194 | |
| Number of reflections | 81156 | |
| <I/σ(I)> | 10.7 | 3.3 |
| Completeness [%] | 90.2 | 91.8 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.8 M Ammonium Sulfate, 100 mM citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
