2IS5
Crystal structure of 3 residues truncated version of protein NMB1012 from Neisseria meningitides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-13 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.744, 79.789, 65.605 |
| Unit cell angles | 90.00, 105.40, 90.00 |
Refinement procedure
| Resolution | 39.800 - 1.850 |
| R-factor | 0.1983 |
| Rwork | 0.197 |
| R-free | 0.22877 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.436 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.075 | 0.475 |
| Number of reflections | 55262 | |
| <I/σ(I)> | 46.8 | 4.32 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.2 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 20 % PEG 3000, Citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
