2IRT

INITIAL CRYSTALLOGRAPHIC ANALYSES OF A RECOMBINANT INTERLEUKIN-1 RECEPTOR ANTAGONIST PROTEIN

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	72.350, 72.350, 114.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.200
Rwork	0.440

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.066 *
Total number of observations	55341 *
Number of reflections	11831 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	277 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir	PEG4000	18-24 (%(w/v))
3	1	reservoir	sodium PIPES	30 (mM)
4	1	reservoir	dithiothreitol	1 (mM)
5	1	reservoir	sodium azide	0.6 (mM)
6	1	reservoir		200-400 (mM)