2IRM
Crystal structure of mitogen-activated protein kinase kinase kinase 7 interacting protein 1 from Anopheles gambiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.773, 73.773, 74.509 |
| Unit cell angles | 90.00, 116.96, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.22117 |
| Rwork | 0.218 |
| R-free | 0.27736 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j4o modified |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.341 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.047 | 0.230 |
| Number of reflections | 14971 | |
| <I/σ(I)> | 13.4 | 2.4 |
| Completeness [%] | 55.0 | 60 |
| Redundancy | 1.8 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 25% PEG 3350, 0.2M Lithium Sulfate, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
