2IQX
Rat Phosphatidylethanolamine-Binding Protein Containing the S153E Mutation in the Complex with o-Phosphorylethanolamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-27 |
| Detector | SBC-2 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.971, 57.211, 80.886 |
| Unit cell angles | 90.00, 109.69, 90.00 |
Refinement procedure
| Resolution | 32.240 - 2.200 |
| R-factor | 0.18957 |
| Rwork | 0.182 |
| R-free | 0.26104 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bd9 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.374 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.250 | 2.290 |
| High resolution limit [Å] | 2.200 | 2.210 |
| Rmerge | 0.123 | 0.276 |
| Number of reflections | 25715 | |
| <I/σ(I)> | 3.1 | 1.8 |
| Completeness [%] | 99.2 | 94.1 |
| Redundancy | 3.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 277 | 10mM o-phosphorylethamine+ 2mM GTP crystallized in 0.1M Sodium Acetate trihydrate pH 4.5 and 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 4.50 |
