2IQJ

Crystal structure of the GAP domain of SMAP1L (LOC64744) stromal membrane-associated protein 1-like

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-09-20
Detector	RIGAKU RAXIS
Wavelength(s)	1.54180
Spacegroup name	P 1
Unit cell lengths	34.812, 38.513, 47.386
Unit cell angles	90.10, 108.89, 105.13

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.195
Rwork	0.192
R-free	0.24570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2crr
RMSD bond length	0.017
RMSD bond angle	1.358
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.920
High resolution limit [Å]	1.850	3.980	1.850
Rmerge	0.052	0.029	0.381
Number of reflections	17983	1890	1731
<I/σ(I)>	14.3
Completeness [%]	94.1
Redundancy	3.9	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	25% PEG-3350, 0.2M Ammonium acetate, 0.1M Bis-Tris, pH 6.5, vapor diffusion, sitting drop, temperature 291K