2IQC
Crystal structure of Human FancF Protein that Functions in the Assembly of a DNA Damage Signaling Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-27 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.00830 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 96.386, 96.386, 47.963 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.070 - 2.400 |
| R-factor | 0.19579 |
| Rwork | 0.193 |
| R-free | 0.25549 |
| Structure solution method | SAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.421 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.250 | 0.220 |
| Number of reflections | 9109 | |
| <I/σ(I)> | 10 | 6.2 |
| Completeness [%] | 99.6 | 97 |
| Redundancy | 7.1 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 100 mM Bis/Tris, 200 mM Lithium sulfate, 20-25% PEG 3350, 10% Ethylene glycol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
