2IQ7
Crystal structure of the polygalacturonase from Colletotrichum lupini and its implications for the interaction with polygalacturonase-inhibiting proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.2 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.640, 127.266, 205.828 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.330 - 1.940 |
| R-factor | 0.15407 |
| Rwork | 0.152 |
| R-free | 0.19619 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1czf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.257 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 105.410 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.081 | 0.396 |
| Number of reflections | 165995 | |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 99.1 | 97.8 |
| Redundancy | 9.2 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 294 | 24% PEG3000, 0.1M CHES, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
