2IPR
Origin binding domain of the SV40 large T antigen (residues 131-259). P21 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-09 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.715, 56.633, 59.889 |
| Unit cell angles | 90.00, 97.13, 90.00 |
Refinement procedure
| Resolution | 59.760 - 1.500 |
| R-factor | 0.17901 |
| Rwork | 0.178 |
| R-free | 0.20427 |
| Structure solution method | MIR |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.422 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.760 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.036 | 0.165 |
| Number of reflections | 39574 | |
| <I/σ(I)> | 33.9 | 7.7 |
| Completeness [%] | 98.5 | 94.2 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 300 | 20 mM NaSCN, 10% P4K, 0.1 Tris, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
