2IPM
Crystal structure of a disulfide mutant glucose binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-06 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.190, 36.600, 64.820 |
| Unit cell angles | 90.00, 106.81, 90.00 |
Refinement procedure
| Resolution | 31.430 - 1.120 |
| R-factor | 0.159 |
| Rwork | 0.158 |
| R-free | 0.17900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hph |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.285 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.190 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.063 | 0.263 |
| Number of reflections | 103523 | |
| <I/σ(I)> | 11.2 | 3.5 |
| Completeness [%] | 100.0 | 99.2 |
| Redundancy | 3.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 20% PEG 6000, 50mM HEPES, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
