2IPC
Crystal structure of the translocation ATPase SecA from Thermus thermophilus reveals a parallel, head-to-head dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-03-15 |
Detector | RIGAKU RAXIS V |
Spacegroup name | P 32 |
Unit cell lengths | 168.623, 168.623, 149.758 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.800 |
R-factor | 0.2213 |
Rwork | 0.221 |
R-free | 0.25450 |
Structure solution method | MIR |
RMSD bond length | 0.013 |
RMSD bond angle | 1.860 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.058 | 0.409 |
Number of reflections | 114222 | |
<I/σ(I)> | 17.9 | 4.2 |
Completeness [%] | 97.4 | 92.3 |
Redundancy | 3.2 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 9% PEG4000, 60mM lithium sulfate, 0.2M NaCl, 87.5 mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |