2IOU
Major Tropism Determinant P1 (Mtd-P1) Variant Complexed with Bordetella brochiseptica Virulence Factor Pertactin extracellular domain (Prn-E).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 |
| Unit cell lengths | 413.464, 413.464, 98.859 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.630 - 3.160 |
| R-factor | 0.22533 |
| Rwork | 0.224 |
| R-free | 0.24934 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yu0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.141 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.156 | 3.156 |
| Rmerge | 0.156 | |
| Number of reflections | 166289 | |
| <I/σ(I)> | 6 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.2 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 2M NaCl, 200mM Lithium Sulfate, 100mM Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
