2IOR
Crystal Structure of the N-terminal Domain of HtpG, the Escherichia coli Hsp90, Bound to ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.494, 77.193, 80.479 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.900 - 1.650 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1byq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.226 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.900 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.058 | 0.342 |
| Number of reflections | 29467 | |
| <I/σ(I)> | 21.3 | 3 |
| Completeness [%] | 99.3 | 94.2 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 2-12% PEG4000, 0.5M 1,6-hexanediol, 0.1M NaOAc, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
