2IOL
Crystal structure of the C-terminal MA3 domain of Pdcd4 (mouse); form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97174 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 64.650, 64.650, 164.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.26056 |
| Rwork | 0.259 |
| R-free | 0.29734 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.482 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.053 |
| High resolution limit [Å] | 2.000 | 2.001 |
| Number of reflections | 26640 | |
| <I/σ(I)> | 15.1 | 1.96 |
| Completeness [%] | 98.0 | 95.93 |
| Redundancy | 4.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 1000, 0.1 M HEPES, 5% GLYCEROL, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
