2IOJ
Crystal structure of protein AF1212 from Archaeoglobus fulgidus, Pfam DRTGG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.409, 66.016, 122.392 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.010 - 2.150 |
| R-factor | 0.236 |
| Rwork | 0.230 |
| R-free | 0.26800 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.080 | 0.215 |
| Number of reflections | 14026 | |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 98.4 | 83.3 |
| Redundancy | 19.7 | 16.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1 M Bis-Tris, pH 6.5, 28% PEG, MME 2000, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
