2IOJ

Crystal structure of protein AF1212 from Archaeoglobus fulgidus, Pfam DRTGG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	62.409, 66.016, 122.392
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.010 - 2.150
R-factor	0.236
Rwork	0.230
R-free	0.26800
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.080	0.215
Number of reflections	14026
<I/σ(I)>	11.8
Completeness [%]	98.4	83.3
Redundancy	19.7	16.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	0.1 M Bis-Tris, pH 6.5, 28% PEG, MME 2000, VAPOR DIFFUSION, HANGING DROP, temperature 294K