2IO8
E. coli Bifunctional glutathionylspermidine synthetase/amidase Incomplex with Mg2+ and ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2005-05-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.200, 75.610, 84.440 |
| Unit cell angles | 70.17, 73.92, 77.77 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| Rwork | 0.173 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.613 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.080 |
| Rmerge | 0.095 | 0.348 |
| Number of reflections | 75954 | |
| <I/σ(I)> | 14.8 | 3.4 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 12% PEG 3350, 0.5M magnesium chloride, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, temperature 298K |
