2IO5

Crystal structure of the CIA- histone H3-H4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-21
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	97.361, 104.780, 86.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.190 - 2.700
R-factor	0.24041
Rwork	0.238
R-free	0.29331
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tey PDB ENTRY 1KX3 chain A and B
RMSD bond length	0.012
RMSD bond angle	1.326
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.190	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.085	0.413
Total number of observations		9916
Number of reflections	12148
<I/σ(I)>	7.1	1.8
Completeness [%]	97.3	100
Redundancy	5.4	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1M HEPES, 0.05M cadmium sulfate hydrate, 1.0M sodium acetate tryhydrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K