2IMO
Crystal structure of allantoate amidohydrolase from Escherichia coli at pH 4.6
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9877,0.9792,0.94 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 94.747, 183.923, 48.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.780 - 2.800 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.30200 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.880 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.110 | 0.500 |
| Number of reflections | 19631 | |
| <I/σ(I)> | 6.9 | 2 |
| Completeness [%] | 98.0 | 84.9 |
| Redundancy | 13.1 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.05 M KH2PO4, 20%PEG8000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
