2IML
Crystal structure of a hypothetical protein from Archaeoglobus fulgidus binding riboflavin 5'-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-09-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.042, 103.182, 103.842 |
| Unit cell angles | 90.00, 97.01, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| R-factor | 0.1702 |
| Rwork | 0.169 |
| R-free | 0.19677 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.243 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.069 | 0.240 |
| Number of reflections | 115408 | |
| <I/σ(I)> | 12.5 | 2.8 |
| Completeness [%] | 95.8 | 76.2 |
| Redundancy | 3.5 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 15% MPD, pH 7, VAPOR DIFFUSION, temperature 294K |
