2IMJ
X-ray Crystal Structure of Protein PFL_3262 from Pseudomonas fluorescens. Northeast Structural Genomics Consortium Target PlR14.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97914 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.858, 88.760, 74.704 |
| Unit cell angles | 90.00, 103.26, 90.00 |
Refinement procedure
| Resolution | 33.350 - 1.500 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| R-free | 0.19300 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.350 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.053 | 0.362 |
| Number of reflections | 195382 | |
| <I/σ(I)> | 18.4 | 3.2 |
| Completeness [%] | 0.9 | 97.4 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.1M Sodium acetate, 16% PEG3350, 0.1 M Sodium Cholride, and 5mM DTT, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
