2ILR

Crystal structure of human Fanconi Anemia protein E C-terminal domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-17
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9794, 0.9798, 0.9764
Spacegroup name	P 41 21 2
Unit cell lengths	59.291, 59.291, 140.249
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.750 - 2.000
R-factor	0.19648
Rwork	0.194
R-free	0.24356
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.776
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.750	2.110
High resolution limit [Å]	2.000	2.000
Number of reflections	17665
<I/σ(I)>	8.1
Completeness [%]	98.9	98.7
Redundancy	8.6	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.085M TrisCl, 0.170M Sodium Acetate, 25.5% PEG 4000, 15% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.085M TrisCl, 0.170M Sodium Acetate, 25.5% PEG 4000, 15% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K