2IL9

Crystal Structure of Plautia Stali Intestine Virus Intergenic Region Internal Ribosome Entry Site Ribosomal Binding Domain RNA at 3.1 Angstroms

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-25
Detector	ADSC QUANTUM 210
Wavelength(s)	1.1053,1.1057,1.1004
Spacegroup name	P 63
Unit cell lengths	133.570, 133.570, 160.425
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	16.970 - 3.100
Rwork	0.253
R-free	0.29400
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE (2.03)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	16.970
High resolution limit [Å]	3.100
Number of reflections	26973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	303	15% MPD, 150 mM MgAcetate, 0.5 mM Spermidine; cryoprotected with 20% MPD, 150 mM MgAcetate, 50 mM Na-MES pH 5.6, 5 mM Iridium (III) hexammine, vapor diffusion, sitting drop, temperature 303K
1	VAPOR DIFFUSION, SITTING DROP	5.6	303	15% MPD, 150 mM MgAcetate, 0.5 mM Spermidine; cryoprotected with 20% MPD, 150 mM MgAcetate, 50 mM Na-MES pH 5.6, 5 mM Iridium (III) hexammine, vapor diffusion, sitting drop, temperature 303K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MPD
10	1	3	Na-MES
11	2	1	MPD
12	2	1	MgAcetate
13	2	1	Spermidine
14	2	2	MPD
15	2	2	MgAcetate
16	2	2	Spermidine
17	2	3	isopropanol
18	2	3	MPD
19	2	3	MgAcetate
2	1	1	MgAcetate
20	2	3	Na-MES
3	1	1	Spermidine
4	1	2	MPD
5	1	2	MgAcetate
6	1	2	Spermidine
7	1	3	Iridium (III) hexammine
8	1	3	MPD
9	1	3	MgAcetate