2IKQ
Crystal structure of mouse Sts-1 PGM domain in complex with phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.960, 74.671, 101.279 |
| Unit cell angles | 90.00, 100.79, 90.00 |
Refinement procedure
| Resolution | 99.500 - 2.609 |
| R-factor | 0.2491 |
| Rwork | 0.248 |
| R-free | 0.27515 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1h0q |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.420 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.690 |
| High resolution limit [Å] | 2.609 | 2.609 |
| Rmerge | 0.630 | 0.556 |
| Number of reflections | 25668 | |
| <I/σ(I)> | 30 | 3.2 |
| Completeness [%] | 98.0 | 99.5 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 14% PEG 8000, 0.1 M HEPES, 0.3 M Na Acetate, 0.2 M Na/K phosphate, pH 7.0, VAPOR DIFFUSION, temperature 293K |
