2IKK
Structural Genomics, the crystal structure of the C-terminal domain of Yurk from Bacillus subtilis subsp. subtilis str. 168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.509, 104.704, 40.538 |
| Unit cell angles | 90.00, 118.58, 90.00 |
Refinement procedure
| Resolution | 35.600 - 1.800 |
| R-factor | 0.19612 |
| Rwork | 0.194 |
| R-free | 0.23029 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.201 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.600 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.099 | 0.461 |
| Number of reflections | 27240 | |
| <I/σ(I)> | 37.9 | 3.57 |
| Completeness [%] | 99.1 | 95.3 |
| Redundancy | 8.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.7 | 293 | 1.9M Ammonium sulfate, 0.1M Tris, pH 8.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
