2IJZ
Crystal structure of aminopeptidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2006-09-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 134.678, 134.553, 134.601 |
| Unit cell angles | 60.12, 60.10, 60.16 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.255 |
| Rwork | 0.255 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u6l |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.700 | 2.730 |
| Rmerge | 0.106 | 0.223 |
| Number of reflections | 147805 | |
| Completeness [%] | 87.0 | 83.6 |
| Redundancy | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 0.1M Sodium Citrate, 10% PEG 15K, pH 5.6, VAPOR DIFFUSION, HANGING DROP |
