2IJQ
Crystal structure of protein rrnAC1037 from Haloarcula marismortui, Pfam DUF309
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.710, 57.770, 107.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.960 - 1.880 |
| R-factor | 0.2 |
| Rwork | 0.197 |
| R-free | 0.25559 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.607 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.080 | 0.238 |
| Number of reflections | 48823 | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 89.0 | 60.4 |
| Redundancy | 3.1 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 30% peg 4000, 0.1 M Tris-HCl, 0.2 M Magnesium Chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
