2IJK

Structure of a Rom protein dimer at 1.55 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	105
Detector technology	IMAGE PLATE
Collection date	2006-07-02
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	98.391, 39.364, 27.100
Unit cell angles	90.00, 95.89, 90.00

Refinement procedure

Resolution	48.910 - 1.550
R-factor	0.17181
Rwork	0.169
R-free	0.21616
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rop
RMSD bond length	0.015
RMSD bond angle	1.387
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER (1.2)
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.610
High resolution limit [Å]	1.550	1.550
Rmerge	0.072	0.244
Number of reflections	14338
<I/σ(I)>	11.1	2.7
Completeness [%]	94.8	60.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6		Hanging drop contained 1 uL protein/RNA mixture (0.3 0.4 mM complex) and 1 uL well buffer (50 mM sodium cacodylate pH 6.0, 0.1 M potassium chloride, 25 mM magnesium chloride, and 15% isopropanol)., VAPOR DIFFUSION, HANGING DROP