2IJ9
Crystal Structure of Uridylate Kinase from Archaeoglobus Fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 94.277, 94.277, 186.672 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.28428 |
| Rwork | 0.283 |
| R-free | 0.33021 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2brx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.377 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.093 | 0.502 |
| Number of reflections | 23600 | |
| <I/σ(I)> | 6.3 | 0.5 |
| Completeness [%] | 87.6 | 54.5 |
| Redundancy | 6 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 294 | 100MM SODIUM CITRATE, PH 4.5, 3.5M AMMONIUM SULFATE, 20% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
