2IIC
Calcium bound structure of alpha-11 giardin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Detector technology | CCD |
| Collection date | 2006-07-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 129.300, 45.285, 131.901 |
| Unit cell angles | 90.00, 116.39, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.930 |
| Rwork | 0.264 |
| R-free | 0.30600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.491 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.010 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Rmerge | 0.104 | 0.493 |
| Number of reflections | 30731 | |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 100mM Bis-Tris pH 6.5, 24% PEG 550 MME, 50mM calcium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
