2II9
Anabaena sensory rhodopsin transducer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-12 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.152, 78.842, 70.582 |
| Unit cell angles | 90.00, 106.81, 90.00 |
Refinement procedure
| Resolution | 28.230 - 2.000 |
| R-factor | 0.22 |
| Rwork | 0.215 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.932 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.000 | 28.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.300 | 2.000 |
| Rmerge | 0.081 | 0.032 | 0.357 |
| Number of reflections | 29903 | ||
| <I/σ(I)> | 8.1 | ||
| Completeness [%] | 98.9 | 100 | 92.9 |
| Redundancy | 3.3 | 3.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 100 mM Na2HPO4, 100 mM citric acid, 35% (v/v) 2-propanol, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
