2II8

Anabaena sensory rhodopsin transducer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-13
Spacegroup name	P 21 21 21
Unit cell lengths	72.799, 119.236, 120.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.220 - 2.100
R-factor	0.231
Rwork	0.228
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	1.899
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	62002

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	100 mM sodium acetate, 10% (w/v) PEG 4000, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K