2II7

Anabaena sensory rhodopsin transducer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-16
Spacegroup name	P 21 21 21
Unit cell lengths	73.833, 122.328, 130.131
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
Rwork	0.259
R-free	0.31500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.420
Data reduction software	d*TREK
Data scaling software	d*TREK
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.100	47.100	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.103	0.040	0.581
Total number of observations		14376	14064
Number of reflections	32009
<I/σ(I)>	7.6	19.2	2.3
Completeness [%]	86.5	80.5	89.6
Redundancy	4.41	4.41	4.33

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	100 mM sodium acetate, 10% (w/v) PEG 4000, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K