2II6
Crystal structure of Pasteurella multocida sialyltransferase D141N mutant in open conformation with CMP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2006-01-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.625, 62.049, 61.529 |
| Unit cell angles | 90.00, 113.70, 90.00 |
Refinement procedure
| Resolution | 31.020 - 1.750 |
| R-factor | 0.20278 |
| Rwork | 0.200 |
| R-free | 0.24669 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ex0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.347 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.020 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 35649 | |
| <I/σ(I)> | 9 | 3.9 |
| Completeness [%] | 97.3 | 96.2 |
| Redundancy | 1.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 25% PEG3350, 0.1 M NaCl, 0.1 M HEPES, 0.4% Triton X-100, pH 7.5 |
