2IHZ
Crystal structure of multifunctional sialyltransferase from pasteurella multocida with CMP-3F-Neu5Ac and alpha-lactose bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-21 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.482, 64.532, 64.410 |
| Unit cell angles | 90.00, 99.03, 90.00 |
Refinement procedure
| Resolution | 63.630 - 2.000 |
| R-factor | 0.21276 |
| Rwork | 0.210 |
| R-free | 0.25493 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ex1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.177 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.940 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 33274 | |
| <I/σ(I)> | 10.28 | 2.15 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG MME 2000, 0.2 M NaBr, 40.4% Triton X-100, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
