2IHY
Structure of the Staphylococcus aureus putative ATPase subunit of an ATP-binding cassette (ABC) transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9794672 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.972, 74.832, 68.818 |
| Unit cell angles | 90.00, 102.21, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.191 |
| R-free | 0.23100 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.094 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.037 | 0.332 |
| Number of reflections | 39887 | |
| <I/σ(I)> | 35.2 | 4.5 |
| Completeness [%] | 98.2 | 97.4 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 1.5 M ammonium sulfate, 0.1 M HEPES pH7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
