2IHS
Crystal structure of the B30.2/SPRY domain of GUSTAVUS in complex with a 20-residue VASA peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2006-02-07 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 80.209, 80.209, 159.798 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| Rwork | 0.219 |
| R-free | 0.26000 |
| Starting model (for MR) | PDP ENTRY 2FNJ |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.032 | 0.281 |
| Number of reflections | 26376 | |
| <I/σ(I)> | 37.1 | 3.6 |
| Completeness [%] | 96.2 | 79.9 |
| Redundancy | 6.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 10% (w/v) PEG 8000, 0.2M calcium acetate, 0.1M imidazole (pH 7.0), VAPOR DIFFUSION, HANGING DROP, temperature 295K |
