2IHS

Crystal structure of the B30.2/SPRY domain of GUSTAVUS in complex with a 20-residue VASA peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	200
Detector technology	CCD
Collection date	2006-02-07
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	80.209, 80.209, 159.798
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
Rwork	0.219
R-free	0.26000
Starting model (for MR)	PDP ENTRY 2FNJ
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.032	0.281
Number of reflections	26376
<I/σ(I)>	37.1	3.6
Completeness [%]	96.2	79.9
Redundancy	6.6	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	10% (w/v) PEG 8000, 0.2M calcium acetate, 0.1M imidazole (pH 7.0), VAPOR DIFFUSION, HANGING DROP, temperature 295K