2IHR
RF2 of Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 144.650, 144.650, 54.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| Rwork | 0.269 |
| R-free | 0.31730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gqe |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.680 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.068 | 0.550 |
| Number of reflections | 20499 | |
| <I/σ(I)> | 14.6 | 3.3 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 291 | 1.8 M ammonium formiate, 150 mM sdoium thiocyanate, 100 mM sodium acetate, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
