2IHM
Polymerase mu in ternary complex with gapped 11mer DNA duplex and bound incoming nucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2005-10-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.028, 96.085, 73.090 |
| Unit cell angles | 90.00, 106.55, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | murine TdT - 1JMS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.106 | 0.505 |
| Number of reflections | 36737 | |
| <I/σ(I)> | 16.1 | 2.5 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.095M Na Citrate pH 5.6, 19% ispropanol, 19% PEG4000, and 5% glycerol |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES | ||
| 2 | 1 | 1 | ispropanol | ||
| 3 | 1 | 1 | PEG4000 | ||
| 4 | 1 | 1 | glycerol | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | HEPES | ||
| 7 | 1 | 2 | ispropanol | ||
| 8 | 1 | 2 | PEG4000 | ||
| 9 | 1 | 2 | glycerol |
