2IHE
Crystal structure of wild-type single-stranded DNA binding protein from Thermus aquaticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-21 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.840, 80.670, 56.510 |
| Unit cell angles | 90.00, 124.40, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1se8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.077 | 0.357 |
| Number of reflections | 14759 | |
| <I/σ(I)> | 12.2 | 3.9 |
| Completeness [%] | 96.8 | 95.9 |
| Redundancy | 5.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 293.15 | 100 mM Imidazole, 35% 2-Ethoxyethanol, 200 mM Calcium acetate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K, pH 7.40 |
