2IHC
Crystal structure of the bric-a-brac (BTB) domain of human BACH1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.440, 53.664, 123.891 |
| Unit cell angles | 90.00, 106.15, 90.00 |
Refinement procedure
| Resolution | 33.300 - 2.440 |
| R-factor | 0.24841 |
| Rwork | 0.247 |
| R-free | 0.27770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Molecular replacement search model = pdb entry 1R2B |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.453 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 19702 | |
| Completeness [%] | 99.3 | 99.4 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20 % PEG3350, 200 mM MgCl2, 0.1M Tris buffer, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
