2IGS
Crystal Structure of the Protein of Unknown Function from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-28 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.424, 100.458, 140.845 |
| Unit cell angles | 90.00, 100.33, 90.00 |
Refinement procedure
| Resolution | 28.520 - 2.170 |
| R-factor | 0.19359 |
| Rwork | 0.187 |
| R-free | 0.25017 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.429 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.520 | 2.250 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.104 | 0.597 |
| Number of reflections | 107066 | |
| <I/σ(I)> | 7 | 2.1 |
| Completeness [%] | 96.7 | 84 |
| Redundancy | 7.7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 25% PEG5K MME, 0.2M ammonium sulfate, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
