2IGP

Crystal Structure of Hec1 CH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	98
Detector technology	CCD
Collection date	2006-06-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97973, 0.97986, 0.95370
Spacegroup name	P 21 21 21
Unit cell lengths	40.929, 44.703, 68.036
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.370 - 1.800
R-factor	0.20083
Rwork	0.199
R-free	0.23123
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.430
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.075	0.330
Number of reflections	12129
<I/σ(I)>	26.7	4.25
Completeness [%]	100.0	100
Redundancy	7	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1 M cacodylate, pH 6.5, 22-30% PEG 8K, 0.2 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1 M cacodylate, pH 6.5, 22-30% PEG 8K, 0.2 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 291K