2IGF

CRYSTAL STRUCTURES OF AN ANTIBODY TO A PEPTIDE AND ITS COMPLEX WITH PEPTIDE ANTIGEN AT 2.8 ANGSTROMS

Experimental procedure

Spacegroup name	P 63 2 2
Unit cell lengths	142.500, 142.500, 101.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.800
Rwork	0.220
RMSD bond length	0.018
RMSD bond angle	3.980
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.142 *
Total number of observations	77906 *
Number of reflections	26851 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5.75 *		taken from Stura, E.A. et al(1989). J. Biol. Chem., 264, 15721-15725. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	19-mer peptide	(mg/ml)
3	1	reservoir	monobasic sodium phosphate	(M)
4	1	reservoir	dibasic potassium phosphate	(M)