2IG6

Crystal structure of a nimc/nima family protein (ca_c2569) from clostridium acetobutylicum at 1.80 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.918370, 0.979251, 0.978981
Spacegroup name	P 21 21 21
Unit cell lengths	52.431, 65.305, 108.788
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.861 - 1.800
Rwork	0.168
R-free	0.19800
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.394
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.861	29.830	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.051	0.052	0.408
Total number of observations		760	8952
Number of reflections	33939
<I/σ(I)>	8.9	11.7	1.9
Completeness [%]	96.4	66.2	96.5
Redundancy	3.5	2.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	7	277	3.2M (NH4)2SO4, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K