2IFU

Crystal Structure of a Gamma-SNAP from Danio rerio

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-24
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	83.378, 90.813, 264.173
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.053 - 2.600
R-factor	0.24
Rwork	0.239
R-free	0.26100
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.469
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.053	40.050	2.460
High resolution limit [Å]	2.400	5.920	2.400
Rmerge	0.093	0.068	0.568
Number of reflections	70210
<I/σ(I)>	11.759		1.19
Completeness [%]	88.3	98.5	53.4
Redundancy	9.8	11.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Protein Solution (10 MG/ML protein, 0.250 M sodium chloride, 0.0031 M sodium azide, 0.0003 M TCEP, 0.005 M BisTris pH 7.0) mixed in a 1:1 ratio with the Well Solution (15% PEG 8K, 0.020 M lithium sulfate, 0.066 M PIPES pH 6.5, 0.034 M MOPS pH 7.0) Cryoprotected with Well Solution supplemented with up to 25% ethylene glycol, vapor diffusion, hanging drop, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP		277	Protein Solution (10 MG/ML protein, 0.250 M sodium chloride, 0.0031 M sodium azide, 0.0003 M TCEP, 0.005 M BisTris pH 7.0) mixed in a 1:1 ratio with the Well Solution (15% PEG 8K, 0.020 M lithium sulfate, 0.066 M PIPES pH 6.5, 0.034 M MOPS pH 7.0) Cryoprotected with Well Solution supplemented with up to 25% ethylene glycol, vapor diffusion, hanging drop, temperature 277K