2IFT
Crystal structure of putative methylase HI0767 from Haemophilus influenzae. NESG target IR102.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97928 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.337, 57.873, 47.522 |
| Unit cell angles | 90.00, 107.36, 90.00 |
Refinement procedure
| Resolution | 29.340 - 2.300 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fpo Chain F |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.960 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.096 | 0.434 |
| Number of reflections | 64570 | |
| <I/σ(I)> | 10.88 | 2.4 |
| Completeness [%] | 98.4 | 85.7 |
| Redundancy | 4.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 7.5 | 293 | 19-21% PEG 4000, 0.2 M Ammonium acetate, 0.1 M HEPES, pH 7.5, microbatch under oil, temperature 293K |
