2IF9
Crystal Structure of SV40 T-antigen origin binding domain disulfide-linked dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-15 |
| Detector | ADSC |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 68.463, 63.982, 63.228 |
| Unit cell angles | 90.00, 94.45, 90.00 |
Refinement procedure
| Resolution | 38.630 - 2.586 |
| Rwork | 0.208 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fuf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.463 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.586 | 2.586 |
| Rmerge | 0.126 | 0.215 |
| Number of reflections | 7009 | |
| <I/σ(I)> | 7.7 | 2.4 |
| Completeness [%] | 85.1 | 53.3 |
| Redundancy | 3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 30 % peg 4000, 0.1M sodium citrate (pH 5.6), 0.2 M ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
