2IEY
Crystal Structure of mouse Rab27b bound to GDP in hexagonal space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-22 |
| Detector | ADSC QUAMTUM 4r |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 66.061, 66.061, 379.988 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.500 - 3.180 |
| R-factor | 0.29152 |
| Rwork | 0.289 |
| R-free | 0.34550 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.181 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.05) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.370 | 3.310 |
| High resolution limit [Å] | 3.180 | 3.180 |
| Rmerge | 0.111 | 0.693 |
| Number of reflections | 9142 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 15.8 | 15.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | PEG 10000, Hepes, pH 7.5, VAPOR DIFFUSION, temperature 293K |
