2IEC
Crystal Structure of uncharacterized conserved archael protein from Methanopyrus kandleri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-08-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 60.833, 60.833, 263.945 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.330 |
| R-factor | 0.2066 |
| Rwork | 0.202 |
| R-free | 0.29550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i52 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.722 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.460 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.068 | 0.103 |
| Number of reflections | 21655 | |
| <I/σ(I)> | 34.1 | 24.5 |
| Completeness [%] | 97.6 | 100 |
| Redundancy | 15.3 | 15.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 294 | 100mM Bis-Tris pH 6.5, 500mM magnesium formate dihydrate, VAPOR DIFFUSION, temperature 294K |
