2IDT
Structure of M98Q mutant of amicyanin, Cu(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.410, 55.480, 27.040 |
| Unit cell angles | 90.00, 95.17, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.000 |
| R-factor | 0.124 |
| R-free | 0.15780 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1aac |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.033 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.067 | 0.093 |
| Number of reflections | 35092 | |
| <I/σ(I)> | 31.1 | 7.8 |
| Completeness [%] | 77.7 | 38 |
| Redundancy | 3.9 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 293 | 2.6 M phosphate, pH 5.5, EVAPORATION, temperature 293K |
